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侧流免疫检测试纸条 1

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环介导等温扩增 1

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从H1N1血凝素序列提取的沙门氏菌传递的COBRA-HA1抗原对甲型流感亚型产生广谱保护作用 Article

Ram Prasad Aganja, Amal Senevirathne, Chandran Sivasankar, John Hwa Lee

《工程（英文）》 2024年 第32卷 第1期   页码 42-57 doi: 10.1016/j.eng.2023.08.001

摘要：

A universal vaccine is in high demand to address the uncertainties of antigenic drift and the reduced effectiveness of current influenza vaccines. In this study, a strategy called computationally optimized broadly reactive antigen (COBRA) was used to generate a consensus sequence of the hemagglutinin globular head portion (HA1) of influenza virus samples collected from 1918 to 2021 to trace evolutionary changes and incorporate them into the designed constructs. Constructs carrying different HA1 regions were delivered into eukaryotic cells by Salmonella-mediated bactofection using a Semliki Forest virus RdRp-dependent eukaryotic expression system, pJHL204. Recombinant protein expression was confirmed by Western blot and immunofluorescence assays. Mice immunized with the designed constructs produced a humoral response, with a significant increase in immunoglobulin G (IgG) levels, and a cell-mediated immune response, including a 1.5-fold increase in CD4+ and CD8+ T cells. Specifically, constructs #1 and #5 increased the production of interferon-γ (IFN-γ) producing CD4+ and CD8+ T cells, skewing the response toward the T helper type 1 cell (Th1) pathway. Additionally, interleukin-4 (IL-4)-producing T cells were upregulated 4-fold. Protective efficacy was demonstrated, with up to 4-fold higher production of neutralizing antibodies and a hemagglutination inhibition titer > 40 against the selected viral strains. The designed constructs conferred a broadly protective immune response, resulting in a notable reduction in viral titer and minimal inflammation in the lungs of mice challenged with the influenza A/PR8/34, A/Brisbane/59/2007, A/California/07/2009, KBPV VR-92, and NCCP 43021 strains. This discovery revolutionizes influenza vaccine design and delivery; Salmonella-mediated COBRA-HA1 is a highly effective in vivo antigen presentation strategy. This approach can effectively combat seasonal H1N1 influenza strains and potential pandemic outbreaks.

关键词： COBRA     Influenza A     Salmonella     Vaccine     Broad spectral protection    

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2008—2017年河南省腹泻患者来源单相鼠伤寒沙门菌ST34的基因组流行病学特征研究 Article

穆玉娇, 李瑞超, 杜鹏程, 张沛, 李岩, 崔生辉, Séamus Fanning, 白莉

《工程（英文）》 2022年 第15卷 第8期   页码 34-44 doi: 10.1016/j.eng.2022.05.006

摘要：

单相鼠伤寒沙门菌（Salmonella enterica serovar 4,[5],12:i:-, S. 4,[5],12:i:-）作为鼠伤寒沙门菌的变种，已成为全球公共卫生领域备受关注的新兴血清型

关键词： 单相鼠伤寒沙门菌     ST34     流行     系统发育     中国谱系    

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基于链置换环介导等温扩增的量子点纳米珠标记侧流免疫检测试纸条快速灵敏检测鼠伤寒沙门氏菌 Article

Yuting Shang,Shuzhen Cai,Qinghua Ye,Qingping Wu,Yanna Shao,Xiaoying Qu,Xinran Xiang,Baoqing Zhou,Yu Ding,Moutong Chen,Liang Xue,Honghui Zhu,Jumei Zhang

《工程（英文）》 2022年 第19卷 第12期   页码 62-70 doi: 10.1016/j.eng.2021.03.024

摘要：

快速、灵敏、即时检测致病菌对食品安全至关重要。在这项研究中，开发了一种新的量子点纳米珠标记的侧流免疫检测试纸条（QB-labelled LFIAS）结合链置换环介导的等温扩增（SD-LAMP）技术，用于定量检测鼠伤寒沙门氏菌（ST）。量子点纳米珠（QB）作为荧光报告材料，具有良好的检测效率。在LAMP中使用了可定制的链置换（SD）探针，以提高方法的特异性并防止副产物捕获。检测基于夹心免疫分析法。荧光条读取器测量测试（T）线和对照（C）线的荧光强度（FI）。条带的线性检测范围为每毫升102 ~ 108个菌落形成单位（CFU）。视觉检出限为103 CFU·mL−1，表明该系统的灵敏度是AuNP 标记试纸的10 倍。根据聚合酶链反应（PCR）和SD-LAMP的琼脂糖凝胶输出分析ST 特异性。在食品中检测到ST，回收率为85%~110%。该方法快速、简便，几乎不需要设备，适用于食品中的细菌检测和临床诊断。

关键词： 鼠伤寒沙门氏菌     量子点纳米珠     侧流免疫检测试纸条     环介导等温扩增     链置换探针    

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Bacteria-mediated tumor-targeted delivery of tumstatin (54-132) significantly suppresses tumor growth in mouse model by inhibiting angiogenesis and promoting apoptosis

《医学前沿（英文）》 2022年 第16卷 第6期   页码 873-882 doi: 10.1007/s11684-022-0925-2

摘要： Tumor growth is an angiogenesis-dependent process and accompanied by the formation of hypoxic areas. Tumstatin is a tumor-specific angiogenesis inhibitor that suppresses the proliferation and induces the apoptosis of tumorous vascular endothelial cells. VNP20009, an attenuated Salmonella typhimurium strain, preferentially accumulates in the hypoxic areas of solid tumors. In this study, a novel Salmonella-mediated targeted expression system of tumstatin (VNP-Tum5) was developed under the control of the hypoxia-induced J23100 promoter to obtain anti-tumor efficacy in mice. Treatment with VNP-Tum5 effectively suppressed tumor growth and prolonged survival in the mouse model of B16F10 melanoma. VNP-Tum5 exhibited a higher efficacy in inhibiting the proliferation and inducing the necrosis and apoptosis of B16F10 cells in vitro and in vivo compared with VNP (control). VNP-Tum5 significantly inhibited the proliferation and migration of mouse umbilical vascular endothelial cells to impede angiogenesis. VNP-Tum5 downregulated the expression of anti-vascular endothelial growth factor A, platelet endothelial cell adhesion molecule-1, phosphorylated phosphoinositide 3 kinase, and phosphorylated protein kinase B and upregulated the expression of cleaved-caspase 3 in tumor tissues. This study is the first to use tumstatin-transformed VNP20009 as a tumor-targeted system for treatment of melanoma by combining anti-tumor and anti-angiogenic effects.

关键词： Salmonella VNP20009     tumstatin     B16F10     melanoma     apoptosis     angiogenesis    

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标题 作者 时间 类型 操作

从H1N1血凝素序列提取的沙门氏菌传递的COBRA-HA1抗原对甲型流感亚型产生广谱保护作用

Ram Prasad Aganja, Amal Senevirathne, Chandran Sivasankar, John Hwa Lee

期刊论文

2008—2017年河南省腹泻患者来源单相鼠伤寒沙门菌ST34的基因组流行病学特征研究

穆玉娇, 李瑞超, 杜鹏程, 张沛, 李岩, 崔生辉, Séamus Fanning, 白莉

期刊论文

基于链置换环介导等温扩增的量子点纳米珠标记侧流免疫检测试纸条快速灵敏检测鼠伤寒沙门氏菌

Yuting Shang,Shuzhen Cai,Qinghua Ye,Qingping Wu,Yanna Shao,Xiaoying Qu,Xinran Xiang,Baoqing Zhou,Yu Ding,Moutong Chen,Liang Xue,Honghui Zhu,Jumei Zhang

期刊论文

Bacteria-mediated tumor-targeted delivery of tumstatin (54-132) significantly suppresses tumor growth in mouse model by inhibiting angiogenesis and promoting apoptosis

期刊论文

白莉：新发耐药沙门菌的流行特征和人群暴露风险（2022年8月31日）

2022年10月17日

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